Borrowed genes give mums the blues

Mums are now a flower of a different color. Japanese researchers have added a hint of clear sky to the humble plant’s palette, genetically engineering the first-ever “true blue” chrysanthemum.

“Obtaining blue-colored flowers is the Holy Grail for plant breeders,” says Mark Bridgen, a plant breeder at Cornell University. The results are “very exciting.”

Compounds called delphinidin-based anthocyanin pigments are responsible for the natural blues in such flowers as pansies and larkspur. Mums lack those compounds. Instead, the flowers come in a variety of other colors, evoking fiery sunsets, new-fallen snow and all things chartreuse.
In previous attempts to engineer a blue hue in chrysanthemums — and roses and carnations — researchers inserted the gene for a key enzyme that controls production of these compounds, causing them to accumulate. But the resulting blooms skewed more violet-purple than blue.
True blue pigment remained elusive, scientists thought, because its origin was complex; multiple genes have been shown to be involved in its generation. But Naonobu Noda, of the National Agriculture and Food Research Organization in Tsukuba, Japan, and colleagues were surprised to find that inserting only two borrowed genes into chrysanthemums created blue flowers. One gene, from Canterbury bells, got the enzyme process started; the other, from butterfly peas, further tweaked the pigment molecules.

Together, the gene double-team transformed 19 of 32 mums, or 59 percent, of the Taihei variety from having pink or magenta blooms into blue beauties. Additional analyses revealed that the blue color arose because of molecular interactions between the tweaked pigment and certain colorless compounds naturally found in many plants, including chrysanthemums. The two-part method could possibly be used in the production of other blue flowers, the researchers report July 26 in Science Advances.

Gene editing creates virus-free piglets

Pigs are a step closer to becoming organ donors for people.

Researchers used molecular scissors known as CRISPR/Cas9 to snip embedded viruses out of pig DNA. Removing the viruses — called porcine endogenous retroviruses, or PERVs — creates piglets that can’t pass the viruses on to transplant recipients, geneticist Luhan Yang and colleagues report online August 10 in Science.

Yang, a cofounder of eGenesis in Cambridge, Mass., and colleagues had previously sliced 62 PERVs at a time from pig cells grown in the lab (SN: 11/14/15, p. 6). Many of the embedded viruses are already damaged and can’t make copies of themselves to pass on an infection. So in the new study, the researchers removed just 25 viruses that were still capable of infecting other cells.
The team had to overcome several technical hurdles to make PERV-less pig cells that still had the normal number of chromosomes. In a process similar to the one that created Dolly the Sheep (SN: 3/1/97, p. 132), researchers sucked the DNA-containing nuclei from the virus-cleaned cells and injected them into pig eggs. The technique, called somatic cell nuclear transfer, is better known as cloning. Embryos made from the cloned cells were transplanted to sows to develop into piglets.

The process is still not very efficient. Researchers placed 200 to 300 embryos in each of 17 sows. Only 37 piglets were born, and 15 are still living. The oldest are about 4 months old. Such virus-free swine could be a starting point for further genetic manipulations to make pig organs compatible with humans.

What happens in Earth’s atmosphere during an eclipse?

As the moon’s shadow races across North America on August 21, hundreds of radio enthusiasts will turn on their receivers — rain or shine. These observers aren’t after the sun. They’re interested in a shell of electrons hundreds of kilometers overhead, which is responsible for heavenly light shows, GPS navigation and the continued existence of all earthly beings.

This part of the atmosphere, called the ionosphere, absorbs extreme ultraviolet radiation from the sun, protecting life on the ground from its harmful effects. “The ionosphere is the reason life exists on this planet,” says physicist Joshua Semeter of Boston University.
It’s also the stage for brilliant displays like the aurora borealis, which appears when charged material in interplanetary space skims the atmosphere. And the ionosphere is important for the accuracy of GPS signals and radio communication.

This layer of the atmosphere forms when radiation from the sun strips electrons from, or ionizes, atoms and molecules in the atmosphere between about 75 and 1,000 kilometers above Earth’s surface. That leaves a zone full of free-floating negatively charged electrons and positively charged ions, which warps and wefts signals passing through it.
Without direct sunlight, though, the ionosphere stops ionizing. Electrons start to rejoin the atoms and molecules they abandoned, neutralizing the atmosphere’s charge. With fewer free electrons bouncing around, the ionosphere reflects radio waves differently, like a distorted mirror.
We know roughly how this happens, but not precisely. The eclipse will give researchers a chance to examine the charging and uncharging process in almost real time.

“The eclipse lets us look at the change from light to dark to light again very quickly,” says Jill Nelson of George Mason University in Fairfax, Va.

Joseph Huba and Douglas Drob of the U.S. Naval Research Laboratory in Washington, D.C., predicted some of what should happen to the ionosphere in the July 17 Geophysical Research Letters. At higher altitudes, the electrons’ temperature should decrease by 15 percent. Between 150 and 350 kilometers above Earth’s surface, the density of free-floating electrons should drop by a factor of two as they rejoin atoms, the researchers say. This drop in free-floating electrons should create a disturbance that travels along Earth’s magnetic field lines. That echo of the eclipse-induced ripple in the ionosphere may be detectable as far away as the tip of South America.

Previous experiments during eclipses have shown that the degree of ionization doesn’t simply die down and then ramp back up again, as you might expect. The amount of ionization you see seems to depend on how far you are from being directly in the moon’s shadow.

For a project called Eclipse Mob, Nelson and her colleagues will use volunteers around the United States to gather data on how the ionosphere responds when the sun is briefly blocked from the largest land area ever.
About 150 Eclipse Mob participants received a build-it-yourself kit for a small radio receiver that plugs into the headphone jack of a smartphone. Others made their own receivers after the project ran out of kits. On August 21, the volunteers will receive signals from radio transmitters and record the signal’s strength before, during and after the eclipse.
Nelson isn’t sure what to expect in the data, except that it will look different depending on where the receivers are. “We’ll be looking for patterns,” she says. “I don’t know what we’re going to see.”

Semeter and his colleagues will be looking for the eclipse’s effect on GPS signals. They would also like to measure the eclipse’s effects on the ionosphere using smartphones — eventually.

For this year’s solar eclipse, they will observe radio signals using an existing network of GPS receivers in Missouri, and intersperse it with small, cheap GPS receivers that are similar to the kind in most phones. The eclipse will create a big cool spot, setting off waves in the atmosphere that will propagate away from the moon’s shadow. Such waves leave an imprint on the ionosphere that affects GPS signals. The team hopes to combine high-quality data with messier data to lay the groundwork for future experiments to tap into the smartphone crowd.

“The ultimate vision of this project is to leverage all 2 billion smartphones around the planet,” Semeter says. Someday, everyone with a phone could be a node in a global telescope.

If it works, it could be a lifesaver. Similar atmospheric waves were seen radiating from the source of the 2011 earthquake off the coast of Japan (SN Online: 6/16/11). “The earthquake did the sort of thing the eclipse is going to do,” Semeter says. Understanding how these waves form and move could potentially help predict earthquakes in the future.

Does the corona look different when solar activity is high versus when it’s low?

Carbondale, Ill., is just a few kilometers north of the point where this year’s total solar eclipse will linger longest — the city will get two minutes and 38 seconds of total darkness when the moon blocks out the sun. And it’s the only city in the United States that will also be in the path of totality when the next total solar eclipse crosses North America, in 2024 (SN: 8/5/17, p. 32). The town is calling itself the Eclipse Crossroads of America.
“Having a solar eclipse that goes through the entire continent is rare enough,” says planetary scientist Padma Yanamandra-Fisher of the Space Science Institute’s branch in Rancho Cucamonga, Calif. “Having two in seven years is even more rare. And two going through the same city is rarer still.”

That makes Carbondale the perfect spot to investigate how the sun’s atmosphere, or corona, looks different when solar activity is high versus low.

Every 11 years or so, the sun cycles from periods of high magnetic field activity to low activity and back again. The frequency of easy-to-see features — like sunspots on the sun’s visible surface, solar flares and the larger eruptions of coronal mass ejections — cycles, too. But it has been harder to trace what happens to the corona’s streamers, the long wispy tendrils that give the corona its crownlike appearance and originate from the magnetic field.
The corona is normally invisible from Earth, because the bright solar disk washes it out. Even space telescopes that are trained on the sun can’t see the inner part of the corona — they have to block some of it out for their own safety (SN Online: 8/11/17). So solar eclipses are the only time researchers can get a detailed view of what the inner corona, where the streamers are rooted, is up to.
Right now, the sun is in a period of exceptionally low activity. Even at the most recent peak in 2014, the sun’s number of flares and sunspots was pathetically wimpy (SN: 11/2/13, p. 22). During the Aug. 21 solar eclipse, solar activity will still be on the decline. But seven years from now during the 2024 eclipse, it will be on the upswing again, nearing its next peak.

Yanamandra-Fisher will be in Carbondale for both events. This year, she’s teaming up with a crowdsourced eclipse project called the Citizen Continental-America Telescope Eclipse experiment. Citizen CATE will place 68 identical telescopes along the eclipse’s path from Oregon to South Carolina.

As part of a series of experiments, Yanamandra-Fisher and her colleagues will measure the number, distribution and extent of streamers in the corona. Observations of the corona during eclipses going back as far as 1867 suggest that streamers vary with solar activity. During low activity, they tend to be more squat and concentrated closer to the sun’s equator. During high activity, they can get more stringy and spread out.

Scientists suspect that’s because as the sun ramps up its activity, its strengthening magnetic field lets the streamers stretch farther out into space. The sun’s equatorial magnetic field also splits to straddle the equator rather than encircle it. That allows streamers to spread toward the poles and occupy new space.

Although physicists have been studying the corona’s changes for 150 years, that’s still only a dozen or so solar cycles’ worth of data. There is plenty of room for new observations to help decipher the corona’s mysteries. And Yanamandra-Fisher’s group might be the first to collect data from the same point on Earth.

“This is pure science that can be done only during an eclipse,” Yanamandra-Fisher says. “I want to see how the corona changes.”

Some high-temperature superconductors might not be so odd after all

A misfit gang of superconducting materials may be losing their outsider status.

Certain copper-based compounds superconduct, or transmit electricity without resistance, at unusually high temperatures. It was thought that the standard theory of superconductivity, known as Bardeen-Cooper-Schrieffer theory, couldn’t explain these oddballs. But new evidence suggests that the standard theory applies despite the materials’ quirks, researchers report in the Dec. 8 Physical Review Letters.

All known superconductors must be chilled to work. Most must be cooled to temperatures that hover above absolute zero (–273.15° Celsius). But some copper-based superconductors work at temperatures above the boiling point of liquid nitrogen (around –196° C). Finding a superconductor that functions at even higher temperatures — above room temperature — could provide massive energy savings and new technologies (SN: 12/26/15, p. 25). So scientists are intent upon understanding the physics behind known high-temperature superconductors.
When placed in a magnetic field, many superconductors display swirling vortices of electric current — a hallmark of the standard superconductivity theory. But for the copper-based superconductors, known as cuprates, scientists couldn’t find whirls that matched the theory’s predictions, suggesting that a different theory was needed to explain how the materials superconduct. “This was one of the remaining mysteries,” says physicist Christoph Renner of the University of Geneva. Now, Renner and colleagues have found vortices that agree with the theory in a high-temperature copper-based superconductor, studying a compound of yttrium, barium, copper and oxygen.

Vortices in superconductors can be probed with a scanning tunneling microscope. As the microscope tip moves over a vortex, the instrument records a change in the electrical current. Renner and colleagues realized that, in their copper compound, there were two contributions to the current that the probe was measuring, one from superconducting electrons and one from nonsuperconducting ones. The nonsuperconducting contribution was present across the entire surface of the material and masked the signature of the vortices.

Subtracting the nonsuperconducting portion revealed the vortices, which behaved in agreement with the standard superconductivity theory. “That, I think, is quite astonishing; it’s quite a feat,” says Mikael Fogelström of Chalmers University of Technology in Gothenburg, Sweden, who was not involved with the research.
The result lifts some of the fog surrounding cuprates, which have so far resisted theoretical explanation. But plenty of questions still surround the materials, Fogelström says. “It leaves many things still open, but it sort of gives a new picture.”